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anti pjak2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti pjak2
    Anti Pjak2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1457 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pjak2+tyr1007+1008/Phospho-Jak2+(Tyr1007%2F1008)+Antibody/pm41857068-290-36-44
    Average 96 stars, based on 1457 article reviews
    anti pjak2 - by Bioz Stars, 2026-10
    96/100 stars

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    Western Blot:

    Article Title: Regulation of leptin signaling and diet-induced obesity by SEL1L-HRD1 ER-associated degradation in POMC expressing neurons
    Article Snippet: Band density was quantitated using the Image Lab software (v6.0.1, Bio-Rad). .. Antibodies for Western blot were as follows: SEL1L (rabbit, 1:8000, Abclonal; catalog E112049), HRD1 (rabbit, 1:2000, ABclonal; catalog E15102), GRP78 BiP (rabbit, 1:5000, Abcam; catalog ab21685), HSP90 (rabbit, 1:5,000, Santa Cruz Biotechnology Inc.; catalog sc-7947), HSP70 (rabbit, 1:1000, Santa Cruz Biotechnology Inc.; catalog sc-1060), FLAG (mouse, 1:2000, Sigma-Aldrich; catalog F-1804), IRE1α (rabbit, 1:2,000, Cell Signaling Technology; catalog 3294), p-eIF2α (rabbit, 1:2000, Cell Signaling Technology; catalog 3597), eIF2α (rabbit, 1:2000, Cell Signaling Technology; catalog 9722), p-JNK (mouse, 1:2000, Cell Signaling Technology; catalog 9255), JNK (rabbit, 1:1000, Cell Signaling Technology; catalog 9252), PERK (Rabbit, 1:1000, Cell Signaling Technology; catalog 3192), pSTAT3 (Tyr705) (rabbit, 1:1000, Cell Signaling Technology; catalog 9131), STAT3 (rabbit, 1:1000, Cell Signaling Technology; catalog 9132), pJAK2 (Tyr1007/1008) (rabbit, 1:1000, Cell Signaling Technology; catalog 3771), JAK2 (rabbit, 1:1000, ABclonal; catalog A19629), Tubulin (mouse, 1:5000, Santa Cruz Biotechnology Inc.; catalog sc-5286), IκBα (rabbit, 1:1000, Cell Signaling Technology; catalog 9242) and IBA1 (rabbit, 1:1000, Proteintech; catalog 10904-1-AP). .. Secondary antibodies for Western blot were goat anti-rabbit IgG HRP and goat anti-mouse IgG HRP at 1:5,000 from Bio-Rad.

    Article Title: Rapamycin enhances the anti-tumor activity of cabozantinib in cMet inhibitor-resistant hepatocellular carcinoma.
    Article Snippet: Cabozantinib, mainly targeting cMet and vascular endothelial growth factor receptor 2, is the secondline treatment for patients with advanced hepatocellular carcinoma (HCC).. However, the lower response rate and resistance limit its enduring clinical benefit.. In this study, we found that cMet-low HCC cells showed primary resistance to cMet inhibitors, and the combination of cabozantinib and mammalian target of rapamycin (mTOR) inhibitor, rapamycin, exhibited a synergistic inhibitory effect on the in vitro cell proliferation and in vivo tumor growth of these cells.

    Article Title: JAK–STAT Signaling in Inflammatory Breast Cancer Enables Chemotherapy-Resistant Cell States
    Article Snippet: .. Western: STAT3 (Cell Signaling Technology, 9139, AB_331757, 1:1,000), pSTAT3 Tyr705 (Cell Signaling Technology, 9145, AACRJournals.org Cancer Res; 83(2) January 15, 2023 267 D ow nloaded from http://aacrjournals.org/cancerres/article-pdf/83/2/264/3259986/264.pdf by IIT Bom bay user on 05 D ecem ber 2023 AB_2491009, 1:1,000); b-Actin (Merck, A2228, AB_476697, 1:10,000), E-cadherin (BD Biosciences, 610181, AB_397580, 1:2,000); pJAK2 Tyr1007/1008 (Cell Signaling Technology, 3776, AB_2617123 1:1000), JAK2 (Cell Signaling Technology, 3230, AB_2128522, 1:1,000), pJAK1 Tyr1034/1035 (Cell SignalingTechnology, 74129, AB_2799851, 1:1,000), JAK1 (Cell Signaling Technology, 3332, AB_2128499, 1:1,000), pSTAT1 Tyr701 (Cell Signaling Technology, 9167, AB_561284, 1:1,000), STAT1 (Cell Signaling Technology, 14994, AB_2737027, 1:1,000), pSTAT3 Ser727 (Cell Signaling Technology, 9134, AB_331589, 1:1,000), pERK1/2 (Cell Signaling Technology, 9101, AB_331646, 1:1,000), ERK1/2 (Cell Signaling Technology, 9102, AB_330744, 1:1,000), CREB (Cell SignalingTechnology, 9197,AB_331277, 1:1,000),Vimentin (Dako, GA630, AB_2827759, 1:1,000), HRP-conjugated antiMouse IgG (HþL; Thermo Fisher Scientific, 32430, AB_1185566), HRP-conjugated antiRabbit IgG (HþL; Thermo Fisher Scientific, 32460, AB_1185567). .. Immunofluoresence: CD44 (Neomarkers, clone 156–3C11, mouse monoclonal IgG2), CD24 (Neomarkers, clone SN3b, mouse monoclonal IgM), pSTAT3 Tyr705 (Cell Signaling Technology, 9145, AB_2491009,1:1,000), Cl-Casp3 (Cell Signaling Technology, 9661, AB_2341188, 1:100), pHH3 (Abcam, ab5176, AB_304763, 1:200).

    Article Title: SEL1L-HRD1 ER-associated degradation regulates leptin receptor maturation and signaling in POMC neurons in diet-induced obesity
    Article Snippet: Band density was quantitated using 368 the Image Lab software on the ChemiDOC XRS+ system (Bio-Rad). .. 369 Antibodies for Western blot were as follows: SEL1L (rabbit, 1:8000, Abclonal; catalog E112049), 370 HRD1 (rabbit, 1:2000, ABclonal; catalog E15102), GRP78 BiP (rabbit, 1:5000, Abcam; catalog 371 ab21685), HSP90 (rabbit, 1:5,000, Santa Cruz Biotechnology Inc.; catalog sc-7947), FLAG 372 (mouse, 1:2000, Sigma-Aldrich; catalog F-1804), IRE1α (rabbit, 1:2,000, Cell Signaling 373 Technology; catalog 3294), p-eIF2α (rabbit, 1:2000, Cell Signaling Technology; catalog 3597), 374 eIF2α (rabbit, 1:2000, Cell Signaling Technology; catalog 9722), p-JNK (mouse, 1:2000, Cell 375 Signaling Technology; catalog 9255), JNK (rabbit, 1:1000, Cell Signaling Technology; catalog 376 9252), PERK (Rabbit, 1:1000, Cell Signaling Technology; catalog 3192), pSTAT3 (Tyr705) 377 (rabbit, 1:1000, catalog 9131, Cell Signaling Technology), STAT3 (rabbit, 1:1000, Cell Signaling 378 Technology; catalog 9132), pJAK2 (Tyr1007/1008) (rabbit, 1:1000, Cell Signaling Technology; 379 catalog 3771), JAK2 (rabbit, 1:1000, ABclonal; catalog A19629), Tubulin (mouse, 1:5000, Santa 380 Cruz Biotechnology Inc.; catalog sc-5286), IκBα (rabbit, 1:1000, Cell Signaling Technology; 381 catalog 9242) and IBA1 (rabbit, 1:1000, Proteintech; catalog 10904-1-AP) 382 Secondary antibodies for Western blot were goat anti-rabbit IgG HRP and goat anti-mouse IgG 383 HRP at 1:5,000, both from Bio-Rad. ..

    Article Title: JAK–STAT Signaling in Inflammatory Breast Cancer Enables Chemotherapy-Resistant Cell States
    Article Snippet: .. Western: STAT3 (Cell Signaling Technology, 9139, AB_331757, 1:1,000), pSTAT3 Tyr705 (Cell Signaling Technology, 9145, AB_2491009, 1:1,000); β-Actin (Merck, A2228, AB_476697, 1:10,000), E-cadherin (BD Biosciences, 610181, AB_397580, 1:2,000); pJAK2 Tyr1007/1008 (Cell Signaling Technology, 3776, AB_2617123 1:1000), JAK2 (Cell Signaling Technology, 3230, AB_2128522, 1:1,000), pJAK1 Tyr1034/1035 (Cell Signaling Technology, 74129, AB_2799851, 1:1,000), JAK1 (Cell Signaling Technology, 3332, AB_2128499, 1:1,000), pSTAT1 Tyr701 (Cell Signaling Technology, 9167, AB_561284, 1:1,000), STAT1 (Cell Signaling Technology, 14994, AB_2737027, 1:1,000), pSTAT3 Ser727 (Cell Signaling Technology, 9134, AB_331589, 1:1,000), pERK1/2 (Cell Signaling Technology, 9101, AB_331646, 1:1,000), ERK1/2 (Cell Signaling Technology, 9102, AB_330744, 1:1,000), CREB (Cell Signaling Technology, 9197, AB_331277, 1:1,000), Vimentin (Dako, GA630, AB_2827759, 1:1,000), HRP-conjugated antiMouse IgG (H+L; Thermo Fisher Scientific, 32430, AB_1185566), HRP-conjugated anti-Rabbit IgG (H+L; Thermo Fisher Scientific, 32460, AB_1185567). .. Immunofluoresence: CD44 (Neomarkers, clone 156–3C11, mouse monoclonal IgG2), CD24 (Neomarkers, clone SN3b, mouse monoclonal IgM), pSTAT3 Tyr705 (Cell Signaling Technology, 9145, AB_2491009,1:1,000), Cl-Casp3 (Cell Signaling Technology, 9661, AB_2341188, 1:100), pHH3 (Abcam, ab5176, AB_304763, 1:200).



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